Improved method for the construction of cosmid sublibraries from yeast artificial chromosomes.

نویسندگان

  • H W Müller
  • E U Meese
چکیده

Eggleston, S.D. Lauder and W.M. Rehrauer. 1994. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58:401-465. 8.Luckow, B., F. Bunz, B. Stillman, P. Lichter and G. Schutz. 1994. Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans. Mol. Cell. Biol. 14:16261634. 9.Quinn, P.G. and D.K. Granner. 1990. Cyclic AMP-dependent protein kinase regulates transcription of the phosphoenolpyruvate carboxykinase gene but not binding of nuclear factors to the cyclic AMP regulatory element. Mol. Cell. Biol. 10:3357-3364. 10.Rosenberg, H.F. 1993. Bacterial chromosomal contamination of a eukaryotic cDNA library. BioTechniques 15:200-201. 11.Singh, H. 1993. Specific recognition site probes for isolating genes encoding DNAbinding proteins. Methods Enzymol. 218:551567. 12.Stuempfle, K.J., M. Koptides, P.G. Quinn and J. Floros. 1996. In vitro analysis of rat surfactant protein A gene expression. Am. J. Physiol. 270:L504-L516.

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USE OF VECTORETTE AND SUBVECTORETTE PCR FOR THE ISOLATION OF TERMINAL SEQUENCES FROM Y EAST ARTIFICIAL CHROMOSOME (YAC) CLONES

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One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes.

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عنوان ژورنال:
  • BioTechniques

دوره 22 2  شماره 

صفحات  -

تاریخ انتشار 1997